| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dietrick, Tessa J (7)
|
MASSC |
51.44 |
39.72 |
-11.72 |
| 2 |
Ryan, Maxalyn E (7)
|
MADST |
41.80 |
41.00 |
-0.80 |
| 3 |
Tallapragada, Harshitha T (7)
|
MAMSA |
43.51 |
41.96 |
-1.55 |
| 4 |
Foppa, Ashley (7)
|
MASSC |
|
42.68 |
- |
| 5 |
Miller, Amelia E (7)
|
MAGRA |
43.62 |
43.51 |
-0.11 |
| 6 |
Long, Grace N (7)
|
MADST |
47.45 |
44.01 |
-3.44 |
| 7 |
Schlosser, Ava G (7)
|
MADST |
|
45.62 |
- |
| 8 |
Bitting, Morgan M (7)
|
MAAAC |
56.28 |
47.49 |
-8.79 |
| 9 |
Lazar, Piper B (7)
|
MAMARA |
43.86 |
47.52 |
3.66 |
| 10 |
Marks, Riley L (7)
|
MAMSA |
58.73 |
51.41 |
-7.32 |
| 11 |
Cielecki, Jessica (7)
|
MAAAC |
53.31 |
53.38 |
0.07 |
| 12 |
Hung, Danica Hailey T (7)
|
MAACST |
1:02.18 |
53.42 |
-8.76 |
| 13 |
Stankiewicz-Goldsmit, Olivia (7)
|
MAFCA |
|
1:01.37 |
- |
|
Davis, Grace O (7)
|
MASSC |
|
NS |
- |